When fly ash is used as a partial replacement for fine aggregate, it can promote the effective utilization of industrial waste, enhance concrete strength without increasing cement content and help address the global sand shortage. However, limited information is available regarding its influence on the shear performance of high-volume fly ash (HVFA) concrete members. This study further investigates the shear performance of HVFA concrete beams. Six shear-dominant concrete beams with identical rectangular cross sections (150 mm × 250 mm) were fabricated and tested under simply supported condition. The water-to-cement (W/C) ratio of concrete remained 0.65, while 330 kg/m³ of sand was replaced by fly ash. The experimental variables included the steel ratio of shear reinforcement and that of longitudinal rebar, and the anchorage detailing of the rebars at the middle of the beam. Static half-cyclic loading was applied to all specimens to investigate the shear force versus chord rotation responses, as well as the maximum load-carrying capacity. Test results indicated that replacement of sand with fly ash by 330 kg/m3 could ensure concrete compressive strength of the order of 50MPa and over, while its W/C was as high as 0.65. The HVFA concrete beams exhibited the same shear performance and shear strength as the high cement high strength (HCHS) concrete beams. Furthermore, the paper also assessed the applicability and accuracy of design equations of shear strength prescribed in current design codes by comparing the calculated and the experimental results.