Self-drilling screws have become the predominant fastening method for cold-formed steel (CFS) roof trusses due to their quick installation and on-site efficiency. In practice, screw connections in truss systems experience either single-shear or double-shear load transfer, depending on the member configuration. This study investigates the shear behavior of two types of CFS connections: (1) steel sheet connected to channel web members in single shear, and (2) double-wall chord members connected to tube web members, where the load transfer occurs concentrically relative to the truss profile and induces double-shear action. A total of eleven quasi-static tests were conducted on specimens with one and two screw configurations to evaluate load - displacement response up to failure. Initial stiffness, ultimate load capacity, and failure modes were determined experimentally. Observed behavior and measured capacities were examined with respect to screw tilting and bearing limit states defined in the AISI S100-24 specification. The results indicate that connections incorporating tube web members develop 98% to 217% higher load-carrying capacity than single shear connections, although lower initial stiffness was observed in the double-shear configuration. These findings provide experimental insight into shear load transfer mechanisms in commonly used CFS truss connections and establish a basis for improved interpretation of connection behavior in CFS roof truss systems.