In the design of slender steel beam-columns, the moment amplification factor is used to estimate the maximum bending moment. The formulas for evaluating the factor have been presented on the basis of the elastic or elastic-plastic analysis, however the initial deflection of beam-columns is not considered. This paper discusses the effect of initial deflection on the value of the moment amplification factor by performing the analytical work. The analytical model is a simply supported beam-column subjected to constant axial compressive force and end moments. First of all, the equilibrium differential equation which governs the problem is solved and the formula for calculating the bending moment is obtained. In the parametric study, magnitude of the initial deflection, the axial load ratio, the slenderness ratio and the end moment ratio are selected as the parameters. The effects of magnitude of the initial deflection and the end moment ratio on the moment amplification factor are discussed.