It is important to accurately determine the vibration characteristics of wooden buildings to evaluate their seismic performance. This study aims to determine the vibration characteristics of a wooden house at each construction stage and to clarify the factors in their change based on the micro-tremor measurements. The construction process of the wooden house is divided into four stages. In the first stage, the wooden house consists of only columns and beams. In the second stage, the lattice bearing walls are installed in the first stage structure. In the third stage, roofing is added. In the fourth stage, the exterior and interior finishes for the lattice bearing walls are conducted and the entire construction work is completed. Therefore, we conducted micro-tremor measurements a total of four times. Based on the results of the micro-tremor measurements, the stiffness ratio of each element of the wooden house was estimated. The exterior and interior finishes for the lattice load-bearing walls occupy approximately 50–70 % of the overall stiffness and their effect on the stiffness of the wooden house is very large.