A shaking table test on a two-story aluminum alloy space frame model with mortise and tenon joints were carried out. The natural frequency and the damping ratio of the model in two directions were obtained by vibration frequency sweep. Inputting EL-Centro (N-S) 0.1g, 0.2g and 0.4g seismic motion, the strain, displacement and acceleration response of the model structure were investigated. It shows that the mortise and tenon joint frame has lower natural vibration frequency, higher damping ratio, better ductility, and better seismic performance than the corresponding rigid connecting structure.